Инд. авторы:	Solovyev M., Gisbert E.
Заглавие:	Influence of time, storage temperature and freeze/thaw cycles on the activity of digestive enzymes from gilthead sea bream (Sparus aurata)
Библ. ссылка:	Solovyev M., Gisbert E. Influence of time, storage temperature and freeze/thaw cycles on the activity of digestive enzymes from gilthead sea bream (Sparus aurata) // Fish Physiology and Biochemistry. - 2016. - Vol.42. - Iss. 5. - P.1383-1394. - ISSN 0920-1742. - EISSN 1573-5168.
Внешние системы:	PubMed: 27083435; DOI: 10.1007/s10695-016-0226-2; WoS: 000383355700011; SCOPUS: 2-s2.0-84963777396;
Реферат:	eng: In this study, we tested the effects of long-term storage (2 years) at −20 °C and short-term storage (several hours) in ice and freeze/thaw cycles on the activities of pancreatic, gastric and intestinal (brush border and cytosolic) digestive enzymes in a teleost fish species. The results revealed a significant lose in activity of pancreatic (trypsin, chymotrypsin, total alkaline proteases and α-amylase) and intestinal cytosolic (leucine–alanine peptidase) enzymes between 140 and 270 days of storage at −20 °C, whereas in contrast, the activity of all the assayed brush border enzymes remained constant during the first 2 years of storage at −20 °C. During short-term storage conditions, the most stable enzymes assayed were those of the enterocytes of the brush border, which did not show any change in activity after being held for 5 h in ice. Five freezing and thawing cycles did not affect the activity of the intestinal brush border enzymes and the cytosolic ones, whereas the activity of trypsin, α-amylase and bile-salt-activated lipase was significantly affected by the number of freezing and thawing cycles. No changes in pepsin activity were found in samples exposed to 1 and 2 freezing and thawing cycles. © 2016, Springer Science+Business Media Dordrecht.
Ключевые слова:	enzymology; intestine; metabolism; microvillus; pancreas; sea bream; stomach; temperature; Animals; Digestion; Enzyme Stability; Hydrolases; Intestines; Microvilli; Pancreas; Sea Bream; Stomach; Temperature; Brush border and cytosolic enzymes; Freeze/thaw cycles; Pancreatic enzymes; Storage temperature; hydrolase; animal; enzyme stability; digestion;
Издано:	2016
Физ. характеристика:	с.1383-1394