Инд. авторы:	Kasianov N.S., Belousova I.A., Pavlushin S.V., Dubovskiy I.M., Podgwaite J.D., Martemyanov V.V., Bakhvalov S.A.
Заглавие:	The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus
Библ. ссылка:	Kasianov N.S., Belousova I.A., Pavlushin S.V., Dubovskiy I.M., Podgwaite J.D., Martemyanov V.V., Bakhvalov S.A. The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus // PloS ONE. - 2017. - Vol.12. - Iss. 8. - Art.e0183940. - ISSN 1932-6203.
Внешние системы:	PubMed: 28854240; DOI: 10.1371/journal.pone.0183940; WoS: 000408693600068; SCOPUS: 2-s2.0-85028530390;
Реферат:	eng: Host innate immunity is one of the factors that determines the resistance of insects to their entomopathogens. In the research reported here we studied whether or not phenoloxidase (PO), a key enzyme in the melanogenesis component of humoral immunity of insects, plays a role in the protection of Lymantria dispar larvae from infection by L. dispar multiple nucleopolyhedrovirus. We studied two types of viral infection: overt and covert. The following lines of investigation were tested: i) the intravital individual estimation of baseline PO activity in haemolymph plasma followed by virus challenging; ii) the specific inhibition of PO activity in vivo by peroral treatment of infected larvae with phenylthiourea (PTU), a competitive inhibitor of PO; iii) the evaluation of PO activity in the haemolymph plasma after larval starvation. Starvation is a stress that activates the covert infection to an overt form. All of these experiments did not show a relationship between PO activity in haemolymph plasma of L. dispar larvae and larval susceptibility to baculovirus. Moreover, starvation-induced activation of covert viral infection to an overt form occurred in 70 percent of virus-carrying larvae against the background of a dramatic increase of PO activity in haemolymph plasma in the insects studied. Our conclusion is that in L. dispar larvae PO activity is not a predictor of host resistance to baculovirus. © 2017 Kasianov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Ключевые слова:	monophenol monooxygenase; monophenol monooxygenase; Article; Baculoviridae; controlled study; DNA virus infection; enzyme activity; enzyme blood level; enzyme inhibition; enzyme specificity; experimental study; hemolymph; host resistance; humoral immunity; in vivo study; infection prevention; infection sensitivity; insect resistance; larva; Lymantria dispar; melanogenesis; monophenol monooxygenase blood level; nonhuman; prediction; starvation; virus carrier; animal; Baculoviridae; enzymology; hemolymph; host pathogen interaction; metabolism; moth; physiology; virology; Animals; Hemolymph; Host-Pathogen Interactions; Monophenol Monooxygenase; Moths; Nucleopolyhedrovirus;
Издано:	2017